Biological Phosphoryl-Transfer Reactions: Understanding Mechanism and Catalysis
نویسندگان
چکیده
منابع مشابه
Biological phosphoryl-transfer reactions: understanding mechanism and catalysis.
Phosphoryl-transfer reactions are central to biology. These reactions also have some of the slowest nonenzymatic rates and thus require enormous rate accelerations from biological catalysts. Despite the central importance of phosphoryl transfer and the fascinating catalytic challenges it presents, substantial confusion persists about the properties of these reactions. This confusion exists desp...
متن کاملEnzyme-catalyzed phosphoryl transfer reactions.
PERSPECTIVES AND SUMMARY ................... ... ...................................................... 877 Classes of enzyme involving reactions at phosphorus...................................................... 878 COVALENT REACTION INTERMEDIATES, CRYPTIC AND OTHERWISE........................................................................................................ 879 Reactions of Pho...
متن کاملModels for biological phosphoryl transfer.
Quantifying the rate of phosphate monoester dianion hydrolysis under physiological conditions has implications for designing transition state mimics and understanding how catalysis is facilitated. Catalysis is energetically most efficient if the mechanistic pathway in solution is stabilised. Monoesters are believed to have a "dissociative" transition state that has little bonding to the nucleop...
متن کاملEnvironmental effects on phosphoryl group bonding probed by vibrational spectroscopy: implications for understanding phosphoryl transfer and enzymatic catalysis.
We have used vibrational spectroscopy to study bonding in monosubstituted dianionic phosphates, both to learn more about basic properties intrinsic to this important class of biological substrates and to assess the ability of vibrational spectroscopy to provide a "sensor" or probe of the local environment experienced by the phosphoryl group. We examined the bonding properties of the phosphoryl ...
متن کاملPhosphoenolpyruvate mutase catalysis of phosphoryl transfer in phosphoenolpyruvate: kinetics and mechanism of phosphorus-carbon bond formation.
Phosphoenolpyruvate phosphomutase (PEP mutase) from Tetrahymena pyriformis catalyzes the rearrangement of phosphoenolpyruvate (PEP) to phosphonopyruvate (P-pyr). A spectrophotometric P-pyr assay consisting of the coupled actions of P-pyr decarboxylase, phosphonoacetaldehyde hydrolase, and alcohol dehydrogenase was devised to monitor mutase catalysis. The reaction constants determined for PEP mu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Annual Review of Biochemistry
سال: 2011
ISSN: 0066-4154,1545-4509
DOI: 10.1146/annurev-biochem-060409-092741